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KMID : 0613820030130050718
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Journal of Life Science
2003 Volume.13 No. 5 p.718 ~ p.722
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Isolation of Alginate-Degrading Marine Bacteria and Characterization of Alginase
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Lee Jae-Hwa
Lee Eun-Yeol
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Abstract
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Various marine microorganisms were isolated from seaweed, and their alginate-degrading activities were investigated. An alginate-degrading bacteria, Vibrio sp. AEBL-211, showed highest level of alginase activity when cultured on a mineral salt medium containing 0.7%(w/v) sodium alginate as the sole carbon source. The intracellular alginase from AEBL-211 was partially purified by ion chromatography on DE 52-cellulose column and gel filteration on Sepacryl G-200 column, and showed guluronate-specific lyase activity.
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KEYWORD
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alginase, alginate, Vibrio sp., guluronate
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